Instruction manual for rat complement 5a (C5a) elisa kit

Rat complement 5a (C5a) elisa kit instruction manual Elisa kit Specification: 48-well configuration / 96-well configuration Standard dilution: 1.5ml × 1 bottle of enzyme label reagent: 3 ml × 1 bottle (48) / 6 ml × 1 bottle (96) [Rat Complement Fragment 5a (C5a) elisa kit] This reagent is for research use only: the concentration of the standard is used as the abscissa, the OD value is the ordinate, and the standard curve is drawn on the coordinate paper. Find the corresponding concentration from the standard curve according to the OD value of the sample; multiply by the dilution factor; or calculate the linear regression equation of the standard curve using the concentration of the standard and the OD value, and substitute the OD value of the sample into the equation to calculate the sample concentration , Multiplied by the dilution factor, which is the actual concentration of the sample. Kit composition: sealing film: 2 pieces (48) / 2 pieces (96) Instructions: 1 sealed bag: 1 standard product: 2700ng / L 0.5ml × 1 bottle 0.5ml × 1 bottle at 2-8 ℃ to store enzyme Standard coated plate: 1 × 48 1 × 96 2-8 ° C Store the sample diluent: 3ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Store the developer A solution: 3ml × 1 bottle 6 ml × 1 bottle 2 -8 ℃ storage of developer B solution: 3ml × 1 bottle 6 ml × 1 bottle 2-8 ℃ storage stop solution: 3ml × 1 bottle 6ml × 1 bottle 2-8 ℃ storage concentrated washing solution: (20ml × 20 times) × 1 bottle (20ml × 30 times) × 1 bottle stored at 2-8 ℃. Experimental principle: This kit uses the double antibody sandwich method to determine the level of rat complement 5a (C5a) in the specimen. Microporous plates were coated with purified rat complement fragment 5a (C5a) antibody to make solid-phase antibodies. Complement fragment 5a (C5a) was added to the monoclonal antibody-coated microwells in turn, followed by HRP labeled complement fragment 5a C5a) The antibody binds to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with the complement segment 5a (C5a) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of rat complement 5a (C5a) in the sample was calculated by a standard curve. Purpose: This kit is used to determine the content of complement 5a (C5a) in rat serum, plasma and related liquid samples. Service commitment: Delivery period: payment to delivery. Free technical consultation and guidance during working hours. Please call to provide customers with sample testing services to maximize the validity of the experimental results (free substitute testing). Storage conditions and expiration date: kit storage: 2-8 ℃ | expiration date: 6 months

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